General description

The p3XFLAG-Myc-CMV -26 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of dual tagged N-terminal 3XFLAG and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The incorporation of 3XFLAG in the expression vector results in increased detection sensitivity using ANTI-FLAG M2 antibody. Efficiency of replication is optimal when using an SV40 T antigen expressing host.

The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3X-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp- Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG^? and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.

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标签：以下资源由Biovector Inc. 质粒载体菌株细胞株基因保藏中心独家供应并发布，侵权必究。Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com质粒 载体 索取 报价 价格 优惠 售价 出售 图谱 序列 酶切位点 转化 构建 求购 购买 订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site

BacMam plasmid vectors 多功能表达载体,昆虫杆状病毒/真核哺乳动物细胞表达/原核表达。哺乳动物细胞重组杆状病毒表达载体
BACMAM VECTOR CONSTRUCTION KIT (Bacmam 2.0 GFP etc.)
Bacmam 2.0 GFP
pThr185?pTyr187
Mitochondria-GFP, BacMam 2.0
Plasma Membrane-GFP, BacMam 2.0
Golgi-RFP, BacMam 2.0
ER-GFP, BacMam 2.0
Actin-RFP, BacMam 2.0
Actin-GFP, BacMam 2.0
Lysosomes-GFP, BacMam 2.0
Peroxisomes-RFP, BacMam 2.0
pTriEx-1.1
pTriEx-1.1 Hygro
pTriEx-1.1 Neo
pTriEx-2
pTriEx-2 Hygro
pTriEx-2 Neo
pTriEx-3
pTriEx-3 Hygro
pTriEx-3 Neo
pTriEx-4
pTriEx-4 Hygro
pTriEx-4 Neo
pTriEx-4 Ek/LIC

pMIG
pQCXIB (w297-1)
pQCXIR (w498-1)
pQCXIZ CMV/TO DEST (w321-2)
pCLNDX
MIEG-hCD4
MSCV PIG (Puro IRES GFP)
MXW-PGK-IRES-EGFP
pBABE HAII
pBabe HcRed
pBABE-puro
pIC112
pIC26
pLIA (CC#1)
pEVX
pMSCV-pBabeMCS-IRES-RFP
pMSCV-TAP
pMSCVpuroATT
pQCXIB empty (w335-1)
pQCXIN CMV/TO DEST (w383-1)
pRXTN
Retrohair
pCLE
pCLXSN
pCLXSN-GFP
pCMMP-MCS-IRES-mRFP
pCMMP-MCS-IRES-Puro
pBp-Control Sponge (CXCR4 sponge)
MDH1-PGK-GFP_2.0
MSCV
MSCV IRES Luciferase
pAC-Luc
pBABE-hygro
BC-EV (empty vector)
pMKO.1 puro
pMSCV PIG (Puro IRES GFP empty vector)
pLJD vector
pOZ-FH-C-puro
pQCXIB CMV/TO DEST (w320-1)