pLenti6.3/V5-DEST慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLenti6.3/V5-DEST
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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载体基本信息
出品公司: | Invitrogen |
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载体名称: | pLenti6.3/V5-DEST |
质粒类型: | 慢病毒表达载体;Gateway系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | Gateway |
载体大小: | 9387 bp |
5' 测序引物及序列: | CMV-F |
3' 测序引物及序列: | WPRE-R |
载体标签: | V5 Epitope Tag |
载体抗性: | Ampicillin |
筛选标记: | Blasticidin |
备注: | The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway? destination vector for expression of a C- terminally V5-tagged protein. |
产品目录号: | V533-06 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 慢病毒 |
载体质粒图谱和多克隆位点信息
载体简介
DescriptionInvitrogen? 's pLenti6.3?V5-DEST? Gateway? Vector Kit is part of our ViraPower? HiPerform? Lentiviral Gateway? Expression Kit (catalog K5330-00). The pLenti6.3?V5-DEST? Gateway? Vector Kit contains the Gateway?-adapted ViraPower? HiPerform? lentiviral expression vector, pLenti6.3?V5-DEST? for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3?V5-DEST? vector is equipped with two key genetic elements, making it a HiPerform? vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.Advantages? Stable expression? Long-term experiments? Accurate titer of functional virus? Flexible and versatile Gateway? recombination cloning technologyKey Features? HiPerform? WPRE and cPPT elements? CMV promoter ? V5 epitope tag at C terminus? Blasticidin selection
载体序列
载体质粒图谱pdf版和相关资料下载
载体应用举例
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