SNU387-Luc cell line人多形性肝细胞癌细胞荧光稳转株

SNU-387 cells stably expressing Luc
Quantity: 10? cells/1.0 ml
Organism: Human
Source Organ: Liver
BioSafety Level: II
Growth Properties: Adherent
Morphology: Epithelial-Like
Population Doubling: 50-80 hours
Propagation: The base medium for this cell line is Prigrow II medium available at NTCC-9TM002. To make the completed growth medium, add the following components to the base medium with the final concentration: 10% fetal bovine serum (NTCC-9TM999), and Penicillin/Streptomycin Solution (NTCC-9G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Freeze Thaw: 1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15 ml sterile conical tube containing 5 ml complete media. 5. Centrifuge cells at 200x g for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO2.
Quality Control: Drug selection by selection marker

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net