mcf-7 LET-R NTCC? cell line Letrozole来曲唑耐药细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：￥498685
- 货 号：MCF7/Let R -1
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector? NTCC? mcf-7 LET-R NTCC? cell line Letrozole来曲唑耐药细胞株
Antigen/Gene or Protein Targets Letrozole resistantParental Line MCF7Host HumanTissue BreastDisease Keywords Breast CancerRelevance The MCF7/LetR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients. However, a major problem is development of resistance against AIs. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI letrozole allows the study of the molecular basis for resistance to AIs to unravel new targets for treatment.Production Details Letrozole-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10?7 M testosterone. A culture of MCF-7 cells were treated with 10?6 M letrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with letrozole. After ~2–3 months, the isolated colonies gave rise to letrozole-resistant cell lines, which could be grown in letrozole-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.Conditional NoResearch Area Cancer, Drug Discovery & DevelopmentGrowth/Phenotype Keywords Breast cancer cell line resistant to the aromatase inhibitor letrozole. Estrogen receptor positive. Progesterone receptor positive when grown in medium without letrozole.Recommended Growing Conditions Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 1 uM letrozole.Notes Human breast cancer cell line derived from MCF-7 cellsOther related cell lines:- LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole- ExeR-1, ExeR-2, ExeR-3 and ExeR-4 resistant to the steroidal AI exemestane- AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozolePassage 436 (AL3117. AL3118)
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
Antigen/Gene or Protein Targets Letrozole resistantParental Line MCF7Host HumanTissue BreastDisease Keywords Breast CancerRelevance The MCF7/LetR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients. However, a major problem is development of resistance against AIs. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI letrozole allows the study of the molecular basis for resistance to AIs to unravel new targets for treatment.Production Details Letrozole-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10?7 M testosterone. A culture of MCF-7 cells were treated with 10?6 M letrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with letrozole. After ~2–3 months, the isolated colonies gave rise to letrozole-resistant cell lines, which could be grown in letrozole-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.Conditional NoResearch Area Cancer, Drug Discovery & DevelopmentGrowth/Phenotype Keywords Breast cancer cell line resistant to the aromatase inhibitor letrozole. Estrogen receptor positive. Progesterone receptor positive when grown in medium without letrozole.Recommended Growing Conditions Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 1 uM letrozole.Notes Human breast cancer cell line derived from MCF-7 cellsOther related cell lines:- LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole- ExeR-1, ExeR-2, ExeR-3 and ExeR-4 resistant to the steroidal AI exemestane- AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozolePassage 436 (AL3117. AL3118)
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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