KCL-22  慢性髓样白血病细胞

KCL-22 Cells

Cat No.: NTCC592181

Cell line:

KCL-22

Species:

human (Homo sapiens)

Cell type:

chronic myeloid leukemia in blast crisis

Origin:

established from the pleural effusion of a 32-year-oldwoman with Philadelphia chromosome-positive CML in blast crisis in 1981;described to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion geneand a p53 mutation

Biosafety level:

1

Permissions and restrictions:

A

Cell Culture Data:

Morphology:

round, single cells in suspension, sometimes in smallclusters;

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

split saturated culture 1:5 to 1:10 every 2-3 days; seedout at ca. 0.5 x 10⁶cells/ml;maintain at ca. 0.2-0.5 x 10⁶cells/ml; maximal density of ca. 2.0 x 10⁶cells/ml

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 24 hours

Harvest:

cell harvest of about 1.0-1.5 x 10⁶cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:

Mycoplasma:

contamination was eliminated with Mycoplasma RemovalAgent (MRA), then negative in microbiological culture, RNA hybridization, PCRassays

Immunology:

CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -,HLA-DR -;

Fingerprint:

multiplex PCR of minisatellite markers revealed a uniqueDNA profile

Species:

confirmed as human by cytogenetics

Cytogenetics:

human hyperdiploid karyotype with 3.3% polyploidy -51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22),t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carriesPhx2 - matches published karyotype

Molec. Genetics:

expression of fusion gene BCR-ABL1 e13-a2 (b2-a2)confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -,MLV -, SMRV -